Cardiac Depressant and Cytotoxic Activities of Hydro Methanoloic Extract of Bacopa monnieri L

Bacopa monnieri is a well known herb for its use in enhancing memory and in treatment of epileptic disorders ever since from ancient times. While effects of hydro-methanolic extracts of Bacopa monnieri on isolated heart has not been reported yet, so in this present study we aim to investigate effect of 75% hydromethanolic extract of Bacopa monnieri on isolated frog heart and cytotoxic activity by using Human MCF-7 cell line. The extract produced significant results by dramatically decreasing the heart rate in a tachycardia induced heart. It shows decrease in the heart rate with an increase in the concentration of dose, showing a negative inotropic and chronotropic effects at 50 μg/ml and 75 μg/ml. Where at 25 μg/ml it showed a significant decrease in the rate of contraction but no change in the heart rate. 1 μg/ml adrenaline was used to induce the tachycardia. The in-vitro cytotoxic study was carried out by following the determination of cell viability by MTT assay on MCF-7 cell line. It produced a significant result with an IC50 median value of 370±0.0 μg/ml. The current study reveals that the hydromethanolic extract of Bacopa monnieri has a significant cardiac depressant activity and also the cytotoxic activity in a dose dependent manner. K. Sai Krishna* 1 , G.Srividya 1 , Konda Ravi Kumar 2 1 Department of Pharmacology, Koringa College of Pharmacy, Korangi, Thallarevu (M). E.G Dist, A.P 2 Department of Pharmaceutical Chemistry, Hindu college of Pharmacy, Amaravathi road, Guntur, A.P., India. Submission: 4 October 2015 Accepted: 9 October 2015 Published: 25 October 2015 www.ijppr.humanjournals.com Citation: K. Sai Krishna et al. Ijppr.Human, 2015; Vol. 4 (3): 140-149. 141